In-Person

  CNLS Conference Room (TA-3, Bldg 1690)

 

Thursday,  Sept. 08,  2022

3:45 – 4:45 pm (MT)

  Light refreshments  3:15 - 3:45 pm



Webex

https://lanl-us.webex.com/lanl-us/j.php?MTID=m69e70ee814373df888942a5ee4066d47


Speaker: Prof. Johannes Schoeneberg

                             UC San Diego                  

                    

“4D Cell Biology: Adaptive optics lattice light-sheet imaging and AI powered big data processing of live stem cell-derived organoids”

 

Abstract: 

4D Cell Biology is now possible, enabled by new methods in stem cell 3D organoid tissue culture, advanced imaging, and big data image analytics for handling and analyzing the resulting gigabytes and terabytes of high-content imaging data. I will illustrate 4D Cell Biology using the two examples of clathrin mediated endocytosis and mitochondrial dynamics. We expressed fluorescent protein fusions of clathrin and dynamin2 at endogenous levels in genome-edited human embryonic stem cells, which were differentiated into hESC-derived intestinal epithelial organoids. We developed the open-source data analysis package pyLattice to do particle tracking in the resulting large 4D data sets. Similarly, we used eGFP fusions with the mitochondria protein Tom20 in induced human pluripotent stem cells (hiPSCs) to capture the mitochondrial network dynamics in 4D. We developed the open-source data analysis package MitoTNT to do first-in-class mitochondrial temporal network tracking.

Bio: 

Professor Schoeneberg oversees the 4D Cell Biology Laboratory https://www.schoeneberglab.org/home in the Departments of Pharmacology and Chemistry & Biochemistry at UC San Diego.  His research interests include measurement of mitochondrial dynamics, lattice light-sheet imaging and machine learning-based lattice light sheet data analytics.